2,287 research outputs found

    Polyamide profiles of porcine milk and of intestinal tissue of pigs during suckling.

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    Previous studies have suggested that luminal polyamines can directly influence intestinal differentiation of neonatal rats. The present investigation has demonstrated the presence of high levels of polyamines in porcine milk and in the intestinal tissues of suckling pigs. The quantities of polyamines in sow's milk sampled between weeks 1 and 8 of lactation were determined using high performance liquid chromatography (HPLC). The concentration of milk spermidine (SPD) remained constant over the first three to four weeks of lactation but increased four-fold between weeks 4 and 7. Neither putrescine nor spermine (SPN) were detected in any of the milk samples. During intestinal development the mucosal SPD/SPN ratio was elevated between weeks 1 and 3, and weeks 5 and 7. The latter period of increase corresponded with the surge in milk SPD concentration. It is suggested that milk SPD is taken up from the intestinal lumen and is involved in potentiating intestinal differentiation during the latter part of the suckling period

    The mixed problem in L^p for some two-dimensional Lipschitz domains

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    We consider the mixed problem for the Laplace operator in a class of Lipschitz graph domains in two dimensions with Lipschitz constant at most 1. The boundary of the domain is decomposed into two disjoint sets D and N. We suppose the Dirichlet data, f_D has one derivative in L^p(D) of the boundary and the Neumann data is in L^p(N). We find conditions on the domain and the sets D and N so that there is a p_0>1 so that for p in the interval (1,p_0), we may find a unique solution to the mixed problem and the gradient of the solution lies in L^p

    Competition and coexistence of bond and charge orders in (TMTTF)2AsF6

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    (TMTTF)2AsF6 undergoes two phase transitions upon cooling from 300 K. At Tco=103 K a charge-ordering (CO) occurs, and at Tsp(B=9 T)=11 K the material undergoes a spin-Peierls (SP) transition. Within the intermediate, CO phase, the charge disproportionation ratio is found to be at least 3:1 from carbon-13 NMR 1/T1 measurements on spin-labeled samples. Above Tsp, up to about 3Tsp, 1/T1 is independent of temperature, indicative of low-dimensional magnetic correlations. With the application of about 0.15 GPa pressure, Tsp increases substantially, while Tco is rapidly suppressed, demonstrating that the two orders are competing. The experiments are compared to results obtained from calculations on the 1D extended Peierls-Hubbard model.Comment: 4 pages, 5 figure

    Impact of high dietary plant protein with or without marine ingredients in gut mucosa proteome of gilthead seabream (Sparus aurata, L.)

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    [EN] The digestive tract, particularly the intestine, represents one of the main sites of interactions with the environment, playing the gut mucosa a crucial role in the digestion and absorption of nutrients, and in the immune defence. Previous researches have proven that the fishmeal replacement by plant sources could have an impact on the intestinal status at both digestive and immune level, compromising relevant productive parameters, such as feed efficiency, growth or survival. In order to evaluate the long-term impact of total fishmeal replacement on intestinal mucosa, the gut mucosa proteome was analysed in fish fed with a fishmeal-based diet, against plant protein-based diets with or without alternative marine sources inclusion. Total fishmeal replacement without marine ingredients inclusion, reported a negative impact in growth and biometric parameters, further an altered gut mucosa proteome. However, the inclusion of a low percentage of marine ingredients in plant protein-based diets was able to maintain the growth, biometrics parameters and gut mucosa proteome with similar values to FM group. A total fishmeal replacement induced a big set of underrepresented proteins in relation to several biological processes such as intracellular transport, assembly of cellular macrocomplex, protein localization and protein catabolism, as well as several molecular functions, mainly related with binding to different molecules and the maintenance of the cytoskeleton structure. The set of downregulated proteins also included molecules which have a crucial role in the maintenance of the normal function of the enterocytes, and therefore, of the epithelium, including permeability, immune and inflammatory response regulation and nutritional absorption. Possibly, the amino acid imbalance presented in VM diet, in a long-term feeding, may be the main reason of these alterations, which can be prevented by the inclusion of 15% of alternative marine sources. Significance: Long-term feeding with plant protein based diets may be considered as a stress factor and lead to a negative impact on digestive and immune system mechanisms at the gut, that can become apparent in a reduced fish performance. The need for fishmeal replacement by alternative ingredients such as plant sources to ensure the sustainability of the aquaculture sector has led the research assessing the intestinal status of fish to be of increasing importance. This scientific work provides further knowledge about the proteins and biologic processes altered in the gut in response to plant protein based diets, suggesting the loss of part of gut mucosa functionality. Nevertheless, the inclusion of alternative marine ingredients was able to reverse these negative effects, showing as a feasible option to develop sustainable aquafeeds.The first author was supported by a contract-grant (Contrato Pre doctoral para la Formacion de Profesorado Universitario) from Subprogramas de Formacion y Movilidad within the Programa Estatal de Promocion del Talento y su Empleabilidad of the Ministerio de Educacion, Cultura y Deporte of Spain.Estruch, G.; Martínez-Llorens, S.; Tomas-Vidal, A.; Monge-Ortiz, R.; Jover Cerda, M.; Brown, PB.; Peñaranda, D. (2020). Impact of high dietary plant protein with or without marine ingredients in gut mucosa proteome of gilthead seabream (Sparus aurata, L.). Journal of Proteomics. 216:1-13. https://doi.org/10.1016/j.jprot.2020.103672S113216Martínez-Llorens, S., Moñino, A. V., Tomás Vidal, A., Salvador, V. J. M., Pla Torres, M., & Jover Cerdá, M. (2007). Soybean meal as a protein source in gilthead sea bream (Sparus aurata L.) diets: effects on growth and nutrient utilization. Aquaculture Research, 38(1), 82-90. doi:10.1111/j.1365-2109.2006.01637.xMoutinho, S., Martínez-Llorens, S., Tomás-Vidal, A., Jover-Cerdá, M., Oliva-Teles, A., & Peres, H. (2017). Meat and bone meal as partial replacement for fish meal in diets for gilthead seabream ( Sparus aurata ) juveniles: Growth, feed efficiency, amino acid utilization, and economic efficiency. Aquaculture, 468, 271-277. doi:10.1016/j.aquaculture.2016.10.024Piccolo, G., Iaconisi, V., Marono, S., Gasco, L., Loponte, R., Nizza, S., … Parisi, G. (2017). Effect of Tenebrio molitor larvae meal on growth performance, in vivo nutrients digestibility, somatic and marketable indexes of gilthead sea bream (Sparus aurata). Animal Feed Science and Technology, 226, 12-20. doi:10.1016/j.anifeedsci.2017.02.007Nengas, I., Alexis, M. N., & Davies, S. J. (1999). High inclusion levels of poultry meals and related byproducts in diets for gilthead seabream Sparus aurata L. Aquaculture, 179(1-4), 13-23. doi:10.1016/s0044-8486(99)00148-9Monge-Ortiz, R., Martínez-Llorens, S., Márquez, L., Moyano, F. J., Jover-Cerdá, M., & Tomás-Vidal, A. (2016). Potential use of high levels of vegetal proteins in diets for market-sized gilthead sea bream (Sparus aurata). Archives of Animal Nutrition, 70(2), 155-172. doi:10.1080/1745039x.2016.1141743Sitjà-Bobadilla, A., Peña-Llopis, S., Gómez-Requeni, P., Médale, F., Kaushik, S., & Pérez-Sánchez, J. (2005). Effect of fish meal replacement by plant protein sources on non-specific defence mechanisms and oxidative stress in gilthead sea bream (Sparus aurata). Aquaculture, 249(1-4), 387-400. doi:10.1016/j.aquaculture.2005.03.031Santigosa, E., Sánchez, J., Médale, F., Kaushik, S., Pérez-Sánchez, J., & Gallardo, M. A. (2008). Modifications of digestive enzymes in trout (Oncorhynchus mykiss) and sea bream (Sparus aurata) in response to dietary fish meal replacement by plant protein sources. Aquaculture, 282(1-4), 68-74. doi:10.1016/j.aquaculture.2008.06.007Kiron, V. (2012). Fish immune system and its nutritional modulation for preventive health care. Animal Feed Science and Technology, 173(1-2), 111-133. doi:10.1016/j.anifeedsci.2011.12.015Minghetti, M., Drieschner, C., Bramaz, N., Schug, H., & Schirmer, K. (2017). A fish intestinal epithelial barrier model established from the rainbow trout (Oncorhynchus mykiss) cell line, RTgutGC. Cell Biology and Toxicology, 33(6), 539-555. doi:10.1007/s10565-017-9385-xGómez, G. D., & Balcázar, J. L. (2008). A review on the interactions between gut microbiota and innate immunity of fish: Table 1. FEMS Immunology & Medical Microbiology, 52(2), 145-154. doi:10.1111/j.1574-695x.2007.00343.xYu, Y., Sitaraman, S., & Gewirtz, A. T. (2004). 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E., … Tocher, D. R. (2012). Effects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar). BMC Genomics, 13(1), 448. doi:10.1186/1471-2164-13-448Martin, S. A. M., Cash, P., Blaney, S., & Houlihan, D. F. (2001). Fish Physiology and Biochemistry, 24(3), 259-270. doi:10.1023/a:1014015530045Martin, S. A. M., Vilhelmsson, O., Médale, F., Watt, P., Kaushik, S., & Houlihan, D. F. (2003). Proteomic sensitivity to dietary manipulations in rainbow trout. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1651(1-2), 17-29. doi:10.1016/s1570-9639(03)00231-0Vilhelmsson, O. T., Martin, S. A. M., Médale, F., Kaushik, S. J., & Houlihan, D. F. (2004). Dietary plant-protein substitution affects hepatic metabolism in rainbow trout (Oncorhynchus mykiss). British Journal of Nutrition, 92(1), 71-80. doi:10.1079/bjn20041176Kumar, G., Hummel, K., Razzazi-Fazeli, E., & El-Matbouli, M. (2019). Modulation of posterior intestinal mucosal proteome in rainbow trout (Oncorhynchus mykiss) after Yersinia ruckeri infection. Veterinary Research, 50(1). doi:10.1186/s13567-019-0673-8Rajan, B., Lokesh, J., Kiron, V., & Brinchmann, M. F. (2013). Differentially expressed proteins in the skin mucus of Atlantic cod (Gadus morhua) upon natural infection with Vibrio anguillarum. BMC Veterinary Research, 9(1). doi:10.1186/1746-6148-9-103Saleh, M., Kumar, G., Abdel-Baki, A.-A., Dkhil, M. A., El-Matbouli, M., & Al-Quraishy, S. (2018). Quantitative shotgun proteomics distinguishes wound-healing biomarker signatures in common carp skin mucus in response to Ichthyophthirius multifiliis. Veterinary Research, 49(1). doi:10.1186/s13567-018-0535-9Saleh, M., Kumar, G., Abdel-Baki, A.-A. S., Dkhil, M. A., El-Matbouli, M., & Al-Quraishy, S. (2019). Quantitative proteomic profiling of immune responses to Ichthyophthirius multifiliis in common carp skin mucus. Fish & Shellfish Immunology, 84, 834-842. doi:10.1016/j.fsi.2018.10.078ENYU, Y.-L., & SHU-CHIEN, A. C. (2011). Proteomics analysis of mitochondrial extract from liver of female zebrafish undergoing starvation and refeeding. Aquaculture Nutrition, 17(2), e413-e423. doi:10.1111/j.1365-2095.2010.00776.xBoonanuntanasarn, S., Nakharuthai, C., Schrama, D., Duangkaew, R., & Rodrigues, P. M. (2019). Effects of dietary lipid sources on hepatic nutritive contents, fatty acid composition and proteome of Nile tilapia (Oreochromis niloticus). Journal of Proteomics, 192, 208-222. doi:10.1016/j.jprot.2018.09.003Ghisaura, S., Anedda, R., Pagnozzi, D., Biosa, G., Spada, S., Bonaglini, E., … Addis, M. F. (2014). Impact of three commercial feed formulations on farmed gilthead sea bream (Sparus aurata, L.) metabolism as inferred from liver and blood serum proteomics. Proteome Science, 12(1). doi:10.1186/s12953-014-0044-3Sabbagh, M., Schiavone, R., Brizzi, G., Sicuro, B., Zilli, L., & Vilella, S. (2019). Poultry by-product meal as an alternative to fish meal in the juvenile gilthead seabream (Sparus aurata) diet. Aquaculture, 511, 734220. doi:10.1016/j.aquaculture.2019.734220Piazzon, M. C., Calduch-Giner, J. A., Fouz, B., Estensoro, I., Simó-Mirabet, P., Puyalto, M., … Pérez-Sánchez, J. (2017). Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets. Microbiome, 5(1). doi:10.1186/s40168-017-0390-3Wulff, T., Petersen, J., Nørrelykke, M. R., Jessen, F., & Nielsen, H. H. (2012). Proteome Analysis of Pyloric Ceca: A Methodology for Fish Feed Development? Journal of Agricultural and Food Chemistry, 60(34), 8457-8464. doi:10.1021/jf3016943Pérez-Sánchez, J., Estensoro, I., Redondo, M. J., Calduch-Giner, J. A., Kaushik, S., & Sitjà-Bobadilla, A. (2013). Mucins as Diagnostic and Prognostic Biomarkers in a Fish-Parasite Model: Transcriptional and Functional Analysis. PLoS ONE, 8(6), e65457. doi:10.1371/journal.pone.0065457Mirghaed, A. T., Yarahmadi, P., Soltani, M., Paknejad, H., & Hoseini, S. M. (2019). Dietary sodium butyrate (Butirex® C4) supplementation modulates intestinal transcriptomic responses and augments disease resistance of rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology, 92, 621-628. doi:10.1016/j.fsi.2019.06.046Estruch, G., Tomás-Vidal, A., El Nokrashy, A. M., Monge-Ortiz, R., Godoy-Olmos, S., Jover Cerdá, M., & Martínez-Llorens, S. (2018). Inclusion of alternative marine by-products in aquafeeds with different levels of plant-based sources for on-growing gilthead sea bream (Sparus aurata, L.): effects on digestibility, amino acid retention, ammonia excretion and enzyme activity. Archives of Animal Nutrition, 72(4), 321-339. doi:10.1080/1745039x.2018.1472408Peres, H., & Oliva-Teles, A. (2009). The optimum dietary essential amino acid profile for gilthead seabream (Sparus aurata) juveniles. 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    Field quantization for open optical cavities

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    We study the quantum properties of the electromagnetic field in optical cavities coupled to an arbitrary number of escape channels. We consider both inhomogeneous dielectric resonators with a scalar dielectric constant ϵ(r)\epsilon({\bf r}) and cavities defined by mirrors of arbitrary shape. Using the Feshbach projector technique we quantize the field in terms of a set of resonator and bath modes. We rigorously show that the field Hamiltonian reduces to the system--and--bath Hamiltonian of quantum optics. The field dynamics is investigated using the input--output theory of Gardiner and Collet. In the case of strong coupling to the external radiation field we find spectrally overlapping resonator modes. The mode dynamics is coupled due to the damping and noise inflicted by the external field. For wave chaotic resonators the mode dynamics is determined by a non--Hermitean random matrix. Upon including an amplifying medium, our dynamics of open-resonator modes may serve as a starting point for a quantum theory of random lasing.Comment: 16 pages, added references, corrected typo

    Multilayered feed forward Artificial Neural Network model to predict the average summer-monsoon rainfall in India

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    In the present research, possibility of predicting average summer-monsoon rainfall over India has been analyzed through Artificial Neural Network models. In formulating the Artificial Neural Network based predictive model, three layered networks have been constructed with sigmoid non-linearity. The models under study are different in the number of hidden neurons. After a thorough training and test procedure, neural net with three nodes in the hidden layer is found to be the best predictive model.Comment: 19 pages, 1 table, 3 figure

    Small scale energy release driven by supergranular flows on the quiet Sun

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    In this article we present data and modelling for the quiet Sun that strongly suggest a ubiquitous small-scale atmospheric heating mechanism that is driven solely by converging supergranular flows. A possible energy source for such events is the power transfer to the plasma via the work done on the magnetic field by photospheric convective flows, which exert drag of the footpoints of magnetic structures. In this paper we present evidence of small scale energy release events driven directly by the hydrodynamic forces that act on the magnetic elements in the photosphere, as a result of supergranular scale flows. We show strong spatial and temporal correlation between quiet Sun soft X-ray emission (from <i>Yohkoh</i> and <i>SOHO</i> MDI-derived flux removal events driven by deduced photospheric flows. We also present a simple model of heating generated by flux submergence, based on particle acceleration by converging magnetic mirrors. In the near future, high resolution soft X-ray images from XRT on the <i>Hinode</i> satellite will allow definitive, quantitative verification of our results

    Magnetic field-dependent interplay between incoherent and Fermi liquid transport mechanisms in low-dimensional tau phase organic conductors

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    We present an electrical transport study of the 2-dimensional (2D) organic conductor tau-(P-(S,S)-DMEDT-TTF)_2(AuBr)_2(AuBr_2)_y (y = 0.75) at low temperatures and high magnetic fields. The inter-plane resistivity rho_zz increases with decreasing temperature, with the exception of a slight anomaly at 12 K. Under a magnetic field B, both rho_zz and the in-plane resistivity plane rho_xx show a pronounced negative and hysteretic magnetoresistance with Shubnikov de Haas (SdH)oscillations being observed in some (high quality)samples above 15 T. Contrary to the predicted single, star-shaped, closed orbit Fermi surface from band structure calculations (with an expected approximate area of 12.5% of A_FBZ), two fundamental frequencies F_l and F_h are detected in the SdH signal. These orbits correspond to 2.4% and 6.8% of the area of the first Brillouin zone(A_FBZ), with effective masses F_l = 4.0 +/- 0.5 and F_h = 7.3 +/- 0.1. The angular dependence, in tilted magnetic fields of F_l and F_h, reveals the 2D character of the FS and Angular dependent magnetoresistance (AMRO) further suggests a FS which is strictly 2-D where the inter-plane hopping t_c is virtually absent or incoherent. The Hall constant R_xy is field independent, and the Hall mobility increases by a factor of 3 under moderate magnetic fields. Our observations suggest a unique physical situation where a stable 2D Fermi liquid state in the molecular layers are incoherently coupled along the least conducting direction. The magnetic field not only reduces the inelastic scattering between the 2D metallic layers, but it also reveals the incoherent nature of interplane transport in the AMRO spectrum. The apparent ferromagnetism of the hysteretic magnetoresistance remains an unsolved problem.Comment: 33 pages, 11 figure
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